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alexa fluor 647 microbeads  (Miltenyi Biotec)


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    Miltenyi Biotec alexa fluor 647 microbeads
    Alexa Fluor 647 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cy5/Anti-Cy5%2FAnti-Alexa+Fluor+647+MicroBeads/pm42414546-275-5-9
    Average 95 stars, based on 53 article reviews
    alexa fluor 647 microbeads - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Bone marrow dendritic cell progenitors sense pathogens via Toll-like receptors and subsequently migrate to inflamed lymph nodes.
    Article Snippet: .. For BM progenitors, cells positive for lineage antigens (Lin; CD3 , CD4, CD8 , CD19, CD11b, B220, Gr-1, NK1.1, and Ter119) were stained with PE-Cy5–conjugated antibodies, labeled with anti–Cy5-MicroBeads (Miltenyi Biotec), and negatively selected with MACS LS-columns and MidiMACS Separators (Miltenyi Biotec). .. For spleen or LN DCs, samples were stained with anti–CD11cAPC, labeled with anti–APC-MicroBeads (Miltenyi Biotec), and enriched for CD11c.

    Labeling:

    Article Title: Bone marrow dendritic cell progenitors sense pathogens via Toll-like receptors and subsequently migrate to inflamed lymph nodes.
    Article Snippet: .. For BM progenitors, cells positive for lineage antigens (Lin; CD3 , CD4, CD8 , CD19, CD11b, B220, Gr-1, NK1.1, and Ter119) were stained with PE-Cy5–conjugated antibodies, labeled with anti–Cy5-MicroBeads (Miltenyi Biotec), and negatively selected with MACS LS-columns and MidiMACS Separators (Miltenyi Biotec). .. For spleen or LN DCs, samples were stained with anti–CD11cAPC, labeled with anti–APC-MicroBeads (Miltenyi Biotec), and enriched for CD11c.

    Magnetic Cell Separation:

    Article Title: Bone marrow dendritic cell progenitors sense pathogens via Toll-like receptors and subsequently migrate to inflamed lymph nodes.
    Article Snippet: .. For BM progenitors, cells positive for lineage antigens (Lin; CD3 , CD4, CD8 , CD19, CD11b, B220, Gr-1, NK1.1, and Ter119) were stained with PE-Cy5–conjugated antibodies, labeled with anti–Cy5-MicroBeads (Miltenyi Biotec), and negatively selected with MACS LS-columns and MidiMACS Separators (Miltenyi Biotec). .. For spleen or LN DCs, samples were stained with anti–CD11cAPC, labeled with anti–APC-MicroBeads (Miltenyi Biotec), and enriched for CD11c.



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    Image Search Results


    BAITs enhance antigen uptake and digestion by DCs under TGFβ stimulation. (A) Schematic illustration of real-time DC migration assay. BAITs in the lower chamber create a chemokine gradient to attract DCs from the upper chamber. (B) Kinetics of DC migration measured as cell index (n = 3; n.s. is P > 0.05, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (C) Crystal violet staining of non-migrated DCs (upper chamber) and Calcein-AM staining of migrated DCs (lower chamber) showing enhanced DC recruitment by BAITs. (D) The antigen uptake efficiency of DCs under varying antigen concentrations with or without TGFβ (n = 5; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (E) Heatmap of immune-related genes in DCs showing upregulation of antigen presentation and co-stimulation markers and downregulation of suppressive factors as antigen concentration increases. (F) Antigen digestion capacity of DCs assessed in the presence or absence of TGFβ (n = 8; ∗∗∗∗ is P < 0.0001 by two-tailed Student's t -test). (G) Fluorescent images showing enhanced antigen uptake in DCs mediated by BAITs (quantified on right; n = 10; ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (H) Flow cytometry analysis of antigen-positive (Cy3 + ) DCs after incubation with free antigen or BAIT (quantified on right; n = 4; ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (I) BAITs improve antigen digestion and uptake of DCs under TGFβ stimulation. DCs were first incubated with Cy5-antigens for 12 h, followed by medium replacement and subsequent treatment with Cy3-antigens or Cy3-BAITs for an additional 6 h. Data are presented as mean ± SD.

    Journal: Bioactive Materials

    Article Title: Countering postoperative immune suppression with a self-assembling dendritic cell nanovaccine

    doi: 10.1016/j.bioactmat.2026.05.005

    Figure Lengend Snippet: BAITs enhance antigen uptake and digestion by DCs under TGFβ stimulation. (A) Schematic illustration of real-time DC migration assay. BAITs in the lower chamber create a chemokine gradient to attract DCs from the upper chamber. (B) Kinetics of DC migration measured as cell index (n = 3; n.s. is P > 0.05, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (C) Crystal violet staining of non-migrated DCs (upper chamber) and Calcein-AM staining of migrated DCs (lower chamber) showing enhanced DC recruitment by BAITs. (D) The antigen uptake efficiency of DCs under varying antigen concentrations with or without TGFβ (n = 5; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (E) Heatmap of immune-related genes in DCs showing upregulation of antigen presentation and co-stimulation markers and downregulation of suppressive factors as antigen concentration increases. (F) Antigen digestion capacity of DCs assessed in the presence or absence of TGFβ (n = 8; ∗∗∗∗ is P < 0.0001 by two-tailed Student's t -test). (G) Fluorescent images showing enhanced antigen uptake in DCs mediated by BAITs (quantified on right; n = 10; ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (H) Flow cytometry analysis of antigen-positive (Cy3 + ) DCs after incubation with free antigen or BAIT (quantified on right; n = 4; ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (I) BAITs improve antigen digestion and uptake of DCs under TGFβ stimulation. DCs were first incubated with Cy5-antigens for 12 h, followed by medium replacement and subsequent treatment with Cy3-antigens or Cy3-BAITs for an additional 6 h. Data are presented as mean ± SD.

    Article Snippet: For MHC I analysis, the cells were incubated with rat CoraLite Plus 488 anti-mouse LAMP1 antibody (1:200) and rabbit anti-mouse MHC I antibody (1:100) overnight at 4 °C, followed by Cy5-conjugated anti-rabbit secondary antibody (1:200) for 1 h at 37 °C.

    Techniques: Migration, Staining, Immunopeptidomics, Concentration Assay, Two Tailed Test, Flow Cytometry, Incubation